脫氧核糖核酸酶 I 來源于牛胰腺

Deoxyribonuclease I from bovine pancreas

別名: DNase I, Deoxyribonuclease, 脫氧核糖核酸 5′-寡核苷酸-水解酶

lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein

CAS號 9003-98-9

orm  ：lyophilized powder

mol wt  ：~31 kDa

composition：Protein, ≥85%

solubility ：0.15 M NaCl: soluble 5.0 mg/mL, hazy

foreign activity  ：RNase ≤0.02%

適用：

Application

? DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA.
? DNAse can be used for Degradation of DNA templates in transcription reactions
? DNAse has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture. 
? Deoxyribonuclease I has also been used along with trypsin for the preparation of single cell suspension from rat testes. 
? Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase.
? Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
? This DNase can be used for the removal of DNA from protein samples.
? Preventing the loss of cells from undesired clumping during tissue dissociation: parts of the cells which are lysed, resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, thereby.

用于從蛋白質樣品中除去 DNA。