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產(chǎn)品名稱:
線性PEI轉(zhuǎn)染試劑(25K)
產(chǎn)品型號(hào):
JX23966
產(chǎn)品展商:
晶欣生物
產(chǎn)品文檔:
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線性PEI轉(zhuǎn)染試劑(25K)
英文名 Polyethylenimine Linear(PEI) MW25000
貨號(hào) JX23966
保存條件 室溫
概述
線性PEI轉(zhuǎn)染試劑是一種陽離子聚合物，分子量25000，它能與核酸形成復(fù)合物，并使該復(fù)合物進(jìn)入哺乳動(dòng)物細(xì)胞。線性PEI轉(zhuǎn)染試劑廣泛適用于常見細(xì)胞系，如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。該試劑即使在有血清存在的情況下，它仍然能高效的將核酸導(dǎo)入細(xì)胞。
我公司提供的線性PEI轉(zhuǎn)染試劑具有如下優(yōu)點(diǎn)：優(yōu)越的轉(zhuǎn)染效率 , 重組蛋白的高表達(dá)水平,與含血清的培養(yǎng)基相兼容, 低細(xì)胞毒性,易于操作。
說明書
Transfection Reagent Preparation and Storage Recommendations
These guidelines are our recommendations based off research, testing, and customer feedback. There are alternative methods available, but we suggest following these recommendations for easy adoption and consistently high performance. These methods are adapted from Cold Spring Harbor Protocols, an excellent secondary resource for transient transfection literature.
Reagent Preparation (1 mg/mL)
Materials:
1.1g PEI 25K (kingmorn Catalog# KE1075).
2.1L Milli-Q® water, water for injection (WFI), or comparable biological-grade water
3.12 M Hydrochloric Acid (HCl)
4.10 M Sodium Hydroxide (NaOH)
5.Disposable 0.1-0.2um PES vacuum sterile-filter.
6.Sterile HDPE or polypropylene storage vials for reagent aliquots.
Equipment
1.1L glass beaker
2.1L glass graduated cylinder
3.Calibrated pH meter
4.2 x disposable 1mL plastic pipettes
5.Stir plate
6.PTFE coated stir bar
7.Vacuum pump
Method:
1.Dispense 1g of PEI 25K into beaker and suspend in 900 mL of water.
2.Add stir bar and set stirring to produce a small vortex.
3.Add hydrochloric acid dropwise until pH is < 2.0.
4.Cover top of beaker and stir for up to 3 hours until powder fully dissolves.
5.Add sodium hydroxide dropwise until pH is 6.9-7.1.
6.Transfer solution to graduated cylinder and add water until the total volume is 1L.
7.Sterile-filter through vacuum membrane.
8.Aliquot as desired and store at -20℃.
Reagent Storage
Frozen aliquots can be stored at -20?C for up to one year. Aliquots can be thawed and kept at 4?C for up to two weeks, but should never be re-frozen.
References
Polyethylenimine (PEI), linear (1 mg/mL). Cold Spring Harbor Protocols 2008, pdb.rec11323– pdb.rec11323 (2008). doi:10.1101/pdb.rec11323
